(A) Expression levels of and after light treatment. The expression analysis was carried out by QRT-PCR. See for light treatments. The results were normalized to the expression of 18S rRNA (internal control), and the expression levels of all genes examined are given relative to the reference value of the transcript level of at 0 h, set to 1. Three independent experiments were performed, and means with standard errors are shown. (B) Expression levels of and in the cotyledon end and the hypocotyl end of lettuce seeds after light treatment. The results were normalized to the expression of 18S rRNA (internal control), and the highest value was set to 100. Two independent experiments were performed, and means with standard errors are shown. (C) Germination frequency of lettuce seeds in the presence of a GA biosynthesis inhibitor and various concentrations of GA. Five sets of 20 decoated lettuce seeds were incubated in the dark at 25 °C in medium containing 50 μM uniconazol-P and various concentrations of GA. After red light treatment, seeds were incubated at 25 °C in the dark for 24 h and the germination frequency was recorded. Means with standard errors are shown.<p><b>Copyright information:</b></p><p>Taken from "Germination of photoblastic lettuce seeds is regulated via the control of endogenous physiologically active gibberellin content, rather than of gibberellin responsiveness"</p><p></p><p>Journal of Experimental Botany 2008;59(12):3383-3393.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2529229.</p><p></p
