Ribosomal Route to Small-Molecule
Diversity
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Abstract
The cyanobactin ribosomal peptide (RP) natural product
pathway
was manipulated to incorporate multiple tandem mutations and non-proteinogenic
amino acids, using eight heterologous components simultaneously expressed
in Escherichia coli. These studies
reveal the potential of RPs for the rational synthesis of complex,
new small molecules over multiple-step biosynthetic pathways using
simple genetic engineering