<p><b>A) Detection of EIF2A phosphorylation following CCT020312 treatment.</b> HT29 human colon cancer and MCF-7 human breast cancer cells were treated with 10 µM CCT020312 (+) or vehicle () for the times indicated. Lysates were prepared and immunoblot-analysis performed using antibodies to detect EIF2A and Ser51-phosphorylated EIF2A (P-S51EIF2A) as indicated. <b>B) Structure-activity relationships.</b> Representatives from different chemical series were tested using HT29 cells for their respective ability to activate EIF2A phosphorylation. <b>C) Analogue structures and potency of analogues for reducing P-S608-pRB and growth.</b></p
