<p>Co-expression of PfnM-GFP and ActM gives rise to a hollow compartment. This enclosure is not dynamically rearranged, as fluorescence does not recover 30 minutes after bleaching (D4, E4, the bleached region is indicate by a red rectangle). Z-sectioning and 3D reconstructions of the cell shown in F. Stepwise rotations along the x-axis of the total enclosure (G1-4) or its “top” half (H1-5) is shown. Immunodetection of ActM in the enclosures of <i>E.coli</i> expressing both ActM and PfnM-GFP reveals a co-localization (J1-3). Images show either GFP-channel (“GFP”), transmission image (“trans”) or an overlay of both (“merge”). Scale bars in Z-sectioning and 3D reconstruction: 1 µm. All other scale bars: 2 µm.</p
