Highly Selective Mitochondria-Targeting
Amphiphilic
Silicon(IV) Phthalocyanines with Axially Ligated Rhodamine B for Photodynamic
Therapy
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Abstract
Two axially ligated rhodamine–Si(IV)–phthalocyanine
(Rh-SiPc) conjugates, bearing one and two rhodamine B, were synthesized
and their linear and two-photon photophysical, subcellular localization
and photocytotoxic properties were studied. These Rh-SiPc conjugates
exhibited an almost exclusive mitochondrial localizing property in
human nasopharyngeal carcinoma (HK-1) cells and human cervical carcinoma
(HeLa) cells. Strong photocytotoxic but low dark cytotoxic properties
were also observed for the two Rh-SiPc conjugates toward the HK-1
cells. Using nuclei staining method and flow cytometric DNA content
analysis, apoptotic cell death was induced by these conjugates upon
photoactivation. This observation is consistent with their mitochondrial
localization property. The observed properties of these conjugates
qualify them as promising PDT agents