<p>(<b>a</b>) Pseudocolor-merged 40x images of a 1:1 mixture of H9 and H9IIIB cells via the slide-based RNA detection method (left) and the suspension method (right) of HIV gag RNA-Alexa Fluor® 546, 18 s rRNA-FITC, and DAPI. The calculated frequencies for HIV gag+18 s+ RNA for each are depicted in each image. (<b>b</b>) Mean intensity comparison of HIV gag for different HIV gag spot count ranges (bins) with the suspension-based and slide-based methods. Error bars depict the standard deviation within each spot count bin. The correlation coefficient, r, and the coefficient of determination, r<sup>2</sup>, are shown on the graph.</p
