Chemical Cross-Linking
and H/D Exchange for Fast Refinement of Protein Crystal
Structure
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Abstract
A combination of chemical cross-linking and hydrogen–deuterium
exchange coupled to high resolution mass spectrometry was used to
describe structural differences of NKR-P1A receptor. The loop region
extended from the compact core in the crystal structure was found
to be closely attached to the protein core in solution. Our approach
has potential to refine protein structures in solution within a few
days and has very low sample consumption