Any unwanted regions for primer design in sequences can be masked using a pair of "" to keep the sequence unchanged

Abstract

Alternatively, the unwanted regions can be replaced with "Ns" (). The included region can be specified by one pair "{}" and only one included region can be masked.<p><b>Copyright information:</b></p><p>Taken from "BatchPrimer3: A high throughput web application for PCR and sequencing primer design"</p><p>http://www.biomedcentral.com/1471-2105/9/253</p><p>BMC Bioinformatics 2008;9():253-253.</p><p>Published online 29 May 2008</p><p>PMCID:PMC2438325.</p><p></p