<p>A) HELFs were electroporated with a mixture of four different small interfering RNAs (siRNA IFI16), scrambled control siRNA (siRNA ctrl), or left not electroporated (NE), and then infected with the indicated viruses at a multiplicity of 0.05 PFU/cell. Cell-free supernatants were harvested on the indicated hours post infection (hpi) and virus amounts determined by plaque assays. The data shown are the average of three experiments ± SD (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA followed by Bonferroni's post test). B) HELFs were transfected with siRNA IFI16 or siRNA ctrl or left not electroporated (NE) and IFI16 expression assayed by Western blotting on the indicated days (d) with anti-IFI16 polyclonal Abs. β-actin was included as a loading control. C) HELFs were treated with siRNA as described for panel A and then infected with HCMV at an MOI of 0.05 (left panel) or 1 (right panel) PFU/cell. Cell-free supernatants were harvested on the indicated hours post infection and the virus amounts determined by plaque assays. The data shown are the average of three experiments ± SD.</p
