Rolling Circle Amplification-Templated
DNA Nanotubes
Show Increased Stability and Cell Penetration Ability
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Abstract
DNA nanotubes hold promise as scaffolds for protein organization,
as templates of nanowires and photonic systems, and as drug delivery
vehicles. We present a new DNA-economic strategy for the construction
of DNA nanotubes with a backbone produced by rolling circle amplification
(RCA), which results in increased stability and templated length.
These nanotubes are more resistant to nuclease degradation, capable
of entering human cervical cancer (HeLa) cells with significantly
increased uptake over double-stranded DNA, and are amenable to encapsulation
and release behavior. As such, they represent a potentially unique
platform for the development of cell probes, drug delivery, and imaging
tools