Quantification of Transcription
Factor Binding in
Cell Extracts Using an Electrochemical, Structure-Switching Biosensor
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Abstract
Transcription factor expression levels, which sensitively
reflect
cellular development and disease state, are typically monitored via
cumbersome, reagent-intensive assays that require relatively large
quantities of cells. Here, we demonstrate a simple, quantitative approach
to their detection based on a simple, electrochemical sensing platform.
This sensor sensitively and quantitatively detects its target transcription
factor in complex media (e.g., 250 μg/mL crude nuclear extracts)
in a convenient, low-reagent process requiring only 10 μL of
sample. Our approach thus appears a promising means of monitoring
transcription factor levels