Detergent-resistant Plasma
Membrane Proteome in Oat
and Rye: Similarities and Dissimilarities between Two Monocotyledonous
Plants
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Abstract
The plasma membrane (PM) is involved in important cellular
processes
that determine the growth, development, differentiation, and environmental
signal responses of plant cells. Some of these dynamic reactions occur
in specific domains in the PM. In this study, we performed comparable
nano-LC–MS/MS-based large-scale proteomic analysis of detergent-resistant
membrane (DRM) fractions prepared from the PM of oat and rye. A number
of proteins showed differential accumulation between the PM and DRM,
and some proteins were only found in the DRM. Numerous proteins were
identified as DRM proteins in oat (219 proteins) and rye (213 proteins),
of which about half were identified only in the DRM. The DRM proteins
were largely common to those found in dicotyledonous plants (<i>Arabidopsis</i> and tobacco), which suggests common functions
associated with the DRM in plants. Combination of semiquantitative
proteomic analysis and prediction of post-translational protein modification
sites revealed differences in several proteins associated with the
DRM in oat and rye. It is concluded that protein distribution in the
DRM is unique from that in the PM, partly because of the physicochemical
properties of the proteins, and the unique distribution of these proteins
may define the functions of the specific domains in the PM in various
physiological processes in plant cells