Representative FACS histograms demonstrating intracellular-location of TLR-4, and a reduced total TLR-4 after CSE treatment.

Abstract

<p>Localisation of Toll-like receptor 4 (TLR-4). (A): Representative histogram for surface and cytoplasmic staining for IgG and TLR-4 in bronchial epithelial cells. (B): rMFI for TLR-4 in permeabilised and non-permeabilised nasal and bronchial epithelial cells. (C): Staining for TLR-4 in permeabilised bronchial epithelial cells after stimulated with 25 µg/ml <i>P aeruginosa</i> LPS, with or without 24 h pre-treatment with CSE. (D): rMFI for TLR-4 was lower in bronchial epithelial cells than for nasal epithelial cells (for control, 4 h and 24 h CSE treatment). A 24 h exposure to CSE significantly decreased rMFI for bronchial, but not for nasal, epithelial cells. Data are displayed as median (interquartile range). <b>*</b> indicates a significant difference (<i>p</i><0.05).</p