Targeted knock-in approach to create new <i>Rosa26</i> MADM with <i>GT</i> and <i>TG</i> cassettes.

Abstract

<p><b>A</b>) Schematic representation of new alleles: <i>R26<sup>GT</sup></i> and <i>R26<sup>TG</sup></i>. <b>B), C) and D)</b> Representative confocal images from tissues indicated on the bottom and genotypes indicated on top. Expected labeling was observed only when Cre was present (compare <b>B</b> with <b>C</b> and <b>D</b>). Bright cellular labeling observed in <b>C</b> and <b>D</b> originates from native tdT and GFP fluorescence (no additional immunostaining was performed). Some sections were stained with DAPI to label nuclei (blue). Scale bars, 50 µm.</p

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