<p><b>A</b>. Cells were starved in media without serum, and treated with synthetic E-peptides at concentrations indicated for 20 minutes. Protein lysates were separated via SDS-PAGE and immunoblotted for Phosphorylated ERK 1 and 2 (P-ERK1/2), stripped, and blotted for Total ERK 1 and 2 (T-ERK1/2). <b>B–C</b>. Quantification of A. <b>D</b>. Cells were treated as above at optimal doses (EA and Scr 1 µM, EB 10 nM) for times indicated. <b>E–F</b> Quantification of C. NoTx at 30 minutes was included in each experiment for normalization between blots. For B–C and E–F, bars represent means ± s.e.m. of N = 3 replicates. *, p<0.05; ***, p<0.001, for comparisons to NoTx via 2-way ANOVA followed by a Bonferroni post-test.</p
