DNA Conjugated SWCNTs
Enter Endothelial Cells via
Rac1 Mediated Macropinocytosis
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Abstract
Several applications of single-walled carbon nanotubes
(SWCNT)
as nanovectors in biological systems have been reported, and several
molecular pathways of cellular entry have been proposed. We employed
transmission electron microscopy, confocal fluorescent microscopy,
and UV–vis spectroscopic analysis to confirm the internalization
of DNA-SWCNT in human umbilical vein endothelial cells. Additionally,
by using pharmacological inhibitors as well as genetic approaches,
we have found that SWCNT is endocytosed through Rac1- GTPase mediated
macropinocytosis in normal endothelial cells