<p>A) Data from the comparison of <i>Tgif1</i> null to wild type P3 MEFs was compared to that from MEFs treated with TGFβ for 10 hours (from GSE15871). Total numbers of probe-sets with significant changes, and the overlaps are shown as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035460#pone-0035460-g005" target="_blank">Figure 5</a>. Chi squared analysis was performed as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035460#pone-0035460-g005" target="_blank">Figure 5</a> and is shown above. B) Data from the comparison of <i>Tgif1</i> null to wild type P3 MEFs was compared to MEFs treated with TNFα for 4 hours (from GSE3700). Data was analyzed and is presented as in A. C) Twelve genes represented in the overlap shown in panel B were analyzed by qRT-PCR. Six genes each were selected from those that went up in both and those that went down in both. Expression was analyzed in triplicate cultures of passage 3 <i>Tgif1</i> null MEFs treated with a TGFβ receptor kinase inhibitor (1 µM SB-431542), or left untreated. Data is shown as mean (+ s.d.) with the value in the untreated cells set to 1 in each case. * p<0.05, ** p<0.01, *** p<0.001, as determined by the Student's T test. Shown below is the fold change (on a linear scale) in the comparison of the <i>Tgif1</i> null to wild type P3 array data for each gene.</p
