Expression levels of CD62L on CD8⁺ splenocytes following primary and recall infection with <i>Lm</i>, <i>L.inn</i> and the <i>L.inn::vgc</i> strain.

Abstract

<p>Flow cytometry was performed on spleen cells, isolated from mice on day 60 after the primary infection or day 5 after the challenge. Cells were stained with FITC-labelled anti-Lyt-2 and biotinylated anti-CD62L. The binding of anti-CD62L on the cell surface was detected with PE-conjugated streptavidin. Numbers shown are gated CD8⁺CD62^lo T cells and analyzed with CELLQuest software.</p