Faculty of Animal Agriculture, Diponegoro University
Abstract
An investigation has been conducted to examine the ratios of inoculums made of buffalo faeces def-ecated
and buffer (artificial saliva) on the analysis of in-vitro digestibility of dry matter and organic matter of
rice straw, corn forage, elephant grass, pangola grass, and peanut straw, respectively. The ratios between
feces to buffer provided were 1:4, 1:2, 2:3, 2:1, and 1:5. Rumen inoculums were obtained from two mature
female rumen-fistulated buffaloes maintaining with mixed ration of 70% elephant grass and 30% concen-trates
fed ad libitum (3% dry matter requirements to body weight). Rumen fluid was collected before
morning feeding. After 10-days acclimatization, the specified data were collected. The results showed that
inoculums ratios of 1 :2 were significantly affected the in-vitro digestibility of forages tested. The efficacity of
this test was 95%. Therefore, faeces and buffer ratios were potential to be used for inoculums for in-vitro
feed digestibility assay during 48 hours incubation period.
Keywords : buffalo, inoculum, faeces, buffer, rumen fluid, in vitr
