<p>(A) A549 cells were stimulated with TGF-β1 and/or TNF-α for 60 min and immunoblotting was performed for total Smad2, phosphorylated Smad2 (linker region: Ser 245/250/255), phosphorylated Smad2 (C-terminal region: Ser 465/467), total Smad3, phosphorylated Smad3 (C-terminal region: Ser 423/425). (B) A549 cells were pretreated with DMSO or chemical inhibitors (LY-364947, U0126, SP600125 and SB203580) for 60 min, followed by TNF-α stimulation. The cell lysates were collected at the indicated time points, and immunoblotting was performed for Smad2, phosphorylated Smad2 (linker region: Ser 245/250/255), Erk, phosphorylated Erk, p38, phosphorylated p38 and phosphorylated c-Jun. α-tubulin was used as loading control.</p
