Strain-Level Typing and
Identification of Bacteria
Using Mass Spectrometry-Based Proteomics
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Abstract
Because of the alarming expansion in the diversity and
occurrence
of bacteria displaying virulence and resistance to antimicrobial agents,
it is increasingly important to be able to detect these microorganisms
and to differentiate and identify closely related species, as well
as different strains of a given species. In this study, a mass spectrometry
proteomics approach is applied, exploiting lipid-based protein immobilization
(LPI), wherein intact bacterial cells are bound, via membrane-gold
interactions, within a FlowCell. The bound cells are subjected to
enzymatic digestion for the generation of peptides, which are subsequently
identified, using LC–MS. Following database matching, strain-specific
peptides are used for subspecies-level discrimination. The method
is shown to enable a reliable typing and identification of closely
related strains of the same bacterial species, herein illustrated
for <i>Helicobacter pylori</i>