<p><b>A</b>) Luciferase reporter assays with the human <i>SPAST</i> promoter. The plasmid pGL3b-SPAST-promoter-Luc (third row) and two deletion derivatives (depicted in the panel on the left) were transfected into Flp-in293 cells and normalized to cells transfected with the pGL3b vector (fourth row). *, <i>P</i><0.05. <b>B</b>) Over-expression of SOX11 upregulates <i>SPAST</i> promoter-reporter constructs having a SOX11 binding site. Flp-In-293 cells were transfected with <i>SPAST</i>-promoter-luciferase reporter constructs both with (blue) and without (red) the SOX11 expression vector. *, <i>P</i><0.05; **, <i>P</i><0.001; ***, <i>P</i><0.0001, and n.s., not statistically significant. Symbols in A and B are as for <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036505#pone-0036505-g001" target="_blank"><b>Fig. 1A</b></a>.</p
