<p><b>A</b>, Cos1-LV1 cells were transiently transfected with Shoc2-tRFP* (S2G) mutant and CFP-H-RAS. Serum-starved cells were incubated with 10 ng/ml EGF-Alexa647 for 12 min at 37°C. Insets show high-magnification images of the regions of the cell indicated by white rectangles. Scale bar, 10 µm. <b>B</b>, High-magnification images of the regions similar to those that are presented in <b>A</b>-insets shown to highlight colocalization of the Shoc2 S2G mutant with EGF and H-RAS. <b>C</b>, Cos-LV1 cells were transiently transfected with Shoc2-tRFP* (S2G) mutant and CFP-Rab5. Serum-starved cells were treated with EGF-Alexa647 as in <b><i>A</i></b>. Insets show high-magnification images of the regions of the cell indicated by white rectangle. Scale bar, 10 µm. <b>D</b>, Cos-LV1 cells were transiently transfected with Shoc2-tRFP* (S2G) mutant and CFP-Rab7. Serum-starved cells were treated with EGF-Alexa647 for 30 min. Insets show high-magnification images of the regions of the cell indicated by white rectangle. Scale bar, 10 µm.</p
