<p>A) Adenosine-independent migration of immature BMDC generated CD73+/+ and CD73−/− in response to AMP. DCs were stimulated with AMP (10<sup>−5</sup> M) or Adenosine (10<sup>−6</sup> M) in the absence or present of ADA (1 IU/ml) for 90 min. Results are shown as chemotatic index, calculated as the number of cells in the lower chamber containing the different stimuli divided by the number of cells in the chamber containing medium alone. One representative out of 3 experiments is shown (n = 3). B) CD73+/+ and CD73−/− BMDCs were incubated with OVA, adenosine (10<sup>−4</sup> M) and AMP in the presence or absence of adenosine deaminase/ADA (1 IU/ml) overnight. Supernatants were collected and IL-10, IL-12, TNF-α concentrations were measured in supernatants by ELISA. Data are means ± SEM of triplicate culture. One representative out of 3 experiments is shown (n = 3).</p
