Conditional expression of <i>RockDN</i> in NCC.

Abstract

<p><b>A</b>) Strategy for expressing <i>RockDN</i> construct in NCC. <b>B</b>) Quantitative real time PCR for the <i>CAT</i> gene cassette, using RNA extracted from pharyngeal arches 1 and 2, from E11.5 mutant <i>RockDN⁺;Wnt1-cre⁺</i> and control embryos. There is a statistically significant (P<0.05 *), 13 fold decrease, in the expression of the <i>CAT</i> box in the mutant sample, as calculated using the one-way Anova test.</p