Abstract

<p>The horizontal bar indicates the DNA sequence map, numbered in kb according to Martin et al. <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002720#pgen.1002720-Martin1" target="_blank">[4]</a> (Genbank U31961). Base #1 corresponds to base 12,809,162 on chromosome 3R in release 5.37 of the <i>Drosophila</i> genome. The coordinates proceed distal to proximal on chromosome 3R, which is opposite in orientation to the whole genome numbering. The regulatory domains <i>iab-2</i> through <i>iab-8</i> are color coded; the domain borders are defined by deletion mutations (<i>Fab8 </i><a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002720#pgen.1002720-Barges1" target="_blank">[<i>22</i>]</a>, ; <i>Fab7</i>, <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002720#pgen.1002720-Gyurkovics1" target="_blank">[41]</a>; <i>Mcp</i>, <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002720#pgen.1002720-Karch3" target="_blank">[44]</a>; <i>iab-3/iab-4</i>, L. Sipos personal communication), or inferred from the binding sites of the CTCF factor <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002720#pgen.1002720-Holohan1" target="_blank">[45]</a>. Below the DNA bar are shown the splicing patterns of <i>abd-A</i> and <i>Abd-B</i> (in black), a cDNA derived from the <i>Mi(Hto-WP)LNP</i> insertion (red), and the MIP06894 cDNA (green). At the bottom, the splicing pattern for the iab-8 ncRNA is shown in dark blue, with numbered exons, and alternate 5′ or 3′ extensions indicated with light blue lines. Mutant lesions are indicated above the DNA bar. The rearrangement breakpoints are color coded according to their phenotypes when heterozygous with the <i>mfs5649</i> insertion.</p

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