<p><b>A</b>) Representative immunoblot showing constitutive phosphorylation and total expression of ERK1/2 in MDA-MB-468 cells with ORAI1 or TRPC1 silencing. <b>B</b>) Densitometric data was obtained from the pooled data (three independent immunoblots) and shows ERK1/2 phosphorylation relative to total ERK1/2 expression; * <i>P</i><0.05 (one-way ANOVA with Bonferroni post-tests). <b>C</b>) DAPI (cell number) and EdU staining (showing cells in S-phase of the cell cycle). <b>D</b>) Quantitation of the average cell count and (<b>E</b>) EdU positivity for nine wells from three independent experiments.* <i>P</i><0.05 (unpaired t-test). All graphs show mean ± S.D. Scale bar represents 100 µm.</p
