AFM images of α-tropomyosin (αTm) molecules.

Abstract

<p>Wildtype human cardiac αTm was imaged dry on poly-lysine coated mica (A). Collage of 20 αTm molecules (B) show that molecular contours were smooth and continuous. One of the αTm molecules in the collage was processed as described (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039676#pone-0039676-g002" target="_blank">Figure 2</a> C) and overlayed with the x-ray structure of αTm <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039676#pone.0039676-Phillips2" target="_blank">[38]</a> on the same scale (B, expanded on right), which is evidence that the AFM images were good representations of single αTm molecules.</p