Complementary Surface-Enhanced
Resonance Raman Spectroscopic
Biodetection of Mixed Protein Solutions by Chitosan- and Silica-Coated
Plasmon-Tuned Silver Nanoparticles
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Abstract
Silver nanoparticles with identical plasmonic properties
but different
surface functionalities are synthesized and tested as chemically selective
surface-enhanced resonance Raman (SERR) amplifiers in a two-component
protein solution. The surface plasmon resonances of the particles
are tuned to 413 nm to match the molecular resonance of protein heme
cofactors. Biocompatible functionalization of the nanoparticles with
a thin film of chitosan yields selective SERR enhancement of the anionic
protein cytochrome <i>b</i><sub>5</sub>, whereas functionalization
with SiO<sub>2</sub> amplifies only the spectra of the cationic protein
cytochrome <i>c</i>. As a result, subsequent addition of
the two differently functionalized particles yields complementary
information on the same mixed protein sample solution. Finally, the
applicability of chitosan-coated Ag nanoparticles for protein separation
was tested by in situ resonance Raman spectroscopy