A minimal concentration of 2.5 µM of TWS119 is required to generate T cells with a young/memory phenotype.

Abstract

<p>Expression of CD45RA and CD62L on CD8⁺ T cells from peripheral blood of healthy donors after activation of the Wnt pathway with TWS119 dose-response (0.5 to 5 µM). (<b>A</b>) Expansion of viable CD8⁺ T cells activated with anti-CD3/IL-2 after five days of culture with or without 5 µM TWS119, illustrated by final cell concentration. Initial cell concentration was 1×10⁶ cell/mL. (<b>B</b>) Representative dot plot analysis for surface expression of CD45RA and CD62L on CD8⁺ T cells activated with anti-CD3/IL-2 with or without increasing concentrations of TWS119. (<b>C</b>) Percentage of total CD8⁺ T cells in each CD45RA/CD62L population for 6 healthy donors. (<b>D</b>) Percentage of CD8⁺ T cells expressing CD127 in total CD8⁺ T cell population in a TWS119 dose-response, in each population defined by expression of CD45RA and CD62L in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041074#pone-0041074-g001" target="_blank">Figure 1A</a> (for 6 donors).</p