<p>(A,B) Spt5 phosphorylation was measured by ChIP using anti-Spt5-P in <i>spt5-myc</i> (MS265; black bars) and <i>spt5-myc htb1-K119R</i> (LV239; gray bars) strains and quantified at the indicated genes by qPCR. Enrichment is plotted as a percentage of input signal for each primer pair. Positions of PCR primer pairs within coding regions are indicated schematically at top. (C,D) Spt5-myc occupancy was measured by ChIP as in A and B. (E,F) Spt5-P enrichment normalized to total Spt5-myc occupancy. (G,H) Cdk9-myc occupancy was measured by ChIP in <i>cdk9-myc</i> (MS264; black bars) and <i>cdk9-myc htb1-K119R</i> (KL259; gray bars) strains. (I,J) H2Bub1 enrichment was measured by ChIP and normalized to H2B-FLAG occupancy. Error bars denote standard deviations from 3 independent experiments. Asterisks denote a significant difference between wild-type and mutant (“*” p<0.04, “**” p<0.02; unpaired t-test).</p
