<p>A, B, immunofluorescence of live MDA-MB 453 and MDA-MB 231 cells respectively, using anti-Cdc37 antibody. Cells were transfected with siRNA targeting Cdc37 (shRNA Cdc37) or with plasmid DNA without any insertion (control). ShRNA Cdc37 transfected cells exhibit extremely low levels of surface immunofluorescence staining as compared to controls. Scale bar = 20 µm. C, D, Western blot analysis of total cell lysates derived from MDA-MB 453 and MDA-MB 231 cells respectively, using the anti-Cdc37 antibody, in cells transfected with siRNA targeting Cdc37 and control cells. The level of Cdc37 detected is significantly lower in both cell lines transfected with siRNA targeting Cdc37, when compared with control transfections.</p
