<p>A) Modulo was depleted in S2 cells by RNAi and chromosome segregation in mitosis was monitored by IF. Staining with Modulo antibody confirmed the successful depletion. Anaphases in cells lacking Modulo (RNAi) displayed lagging and stretched chromosomes at higher frequency than control cells. Bar 5 µm. B) Chromosome segregation was monitored by timelapse microscopy in S2 cells expressing H2B-GFP and mCherry-tubulin. Representative frames for a control and RNAi video are shown. Bar 5 µm. C) Representative anaphases identifiable by H3 Ser10p staining from brain whole-mounts from third instar larvae of <i>mod<sup>lethal8</sup></i> heterozygote mutants (<i>mod/+</i>) and in <i>mod<sup>lethal8</sup></i> homozygote mutants (<i>mod/mod</i>). Frequent stretched and lagging chromosomes were observed in the homozygote mutant (see text for details). Bar 15 µm.</p
