Heatmap displaying the methylation status of CpG loci (n = 297) mapping in 10 selected genes in relation to quality-fractioned sperm populations (i.e. swim-up “up” and swim-down “dn” sperm fractions).
<p>A) The dendrograms above the heatmap show hierarchical clustering based on the methylation data alone. Sperm populations and CpG loci are represented by columns and rows, respectively. Each cell indicates the CpG methylation level for one site in each sample. Methylation levels are represented in the scale on the right side of the heatmap and are referred lowest to highest as green (0.0) to red (1.0). B) Scatter plot reporting CpGs methylation levels between quality-fractioned sperm populations (Up vs Dn) among different individuals. R<sup>2</sup> = Pearson coefficient. C) List of the 10 analyzed genes, selected because previously reported as differently methylated in infertile men compared to normozoospermic controls.</p
