<p><b>A</b> Representative histograms of FITC-fluorescence for rECD-wt binding with 5 mM CaCl<sub>2</sub> (1) or with 2 mM EGTA (2). HT1080 cells were incubated with (black line) or without (negative control, grey filled area) rECD-wt. Bound rECD-wt was detected with anti-HisFITC. <b>B</b> Column plots representing the ratios of rECD-wt-binding (ratio<sub>rECD-bound</sub>; for calculation see Supporting Information) indicated as mean±SEM of 3 independent measurements as detected by flow cytometry. The ratio<sub>rECD-bound</sub> was significantly (p<0.01) decreased from 1.21±0.03 for samples incubated in 5 mM CaCl<sub>2</sub> (1) to 1.05±0.03 for samples incubated with 2 mM EGTA (2) showing that rECD-wt has a Ca<sup>2+</sup>-dependent binding to HT1080 cells. Statistical analysis was performed with unpaired student’s t-test (GraphPad Prism 5.01).</p
