Discovery of Novel Integrin
Ligands from Combinatorial
Libraries Using a Multiplex “Beads on a Bead” Approach
- Publication date
- Publisher
Abstract
The development of screening approaches to identify novel
affinity
ligands has paved the way for a new generation of molecular targeted
nanomedicines. Conventional methods typically bias the display of
the target protein to ligands during the screening process. We have
developed an unbiased multiplex “beads on a bead” strategy
to isolate, characterize, and validate high affinity ligands from
OBOC libraries. Novel non-RGD peptides that target α<sub>v</sub>β<sub>3</sub> integrin were discovered that do not affect cancer
or endothelial cell biology. The peptides identified here represent
novel integrin-targeted agents that can be used to develop targeted
nanomedicines without the risk of increased tumor invasion and metastasis