Construction of a Rhythm
Transfer System That Mimics
the Cellular Clock
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Abstract
Creation of an artificial oscillating gene expression
system is
one of the most challenging issues in synthetic biology. Here, we
constructed a simple system to manipulate gene expression patterns
to be circadian, reflecting the intrinsic cellular clock, by fusing
a core clock protein, BMAL1 or CLOCK, with a zinc finger-type DNA
binding domain. Circadian rhythmic gene expression was induced only
when the target gene contained zinc finger-binding sequences. To our
knowledge, this simple approach is the first to manipulate gene expression
patterns into circadian rhythms and would be applicable to various
endogenous genes