<p>Assessment of inflammatory responses in the CNS of WT (closed squares) or <i>Il-1r<sup>−/−</sup></i> (open squares) mice. Leukocyte infiltration into the CNS was assessed by flow cytometry at days 6–10 post infection (p.i.) with WNV-TX (<b>A–E</b>) as derived from total cell numbers. Cells recovered from perfused whole brain were stained with antibodies to CD45 and CD11b and total cell number (<b>B–E</b>) was assessed by flow cytometry. Representative dot-plot analysis for CD11b and CD45 infiltrates and microglia at mock, day 7 and day 10 p.i. is shown (<b>A</b>). Brains were harvested from tissues of WNV-TX02 infected mice at indicated time-points homogenized and assessed for cytokine and chemokine expression by luminex array (<b>F–I</b>). Data are shown as mean +/− S.E.M. for n = 3–4 per time-point and are compiled from 2–3 independent experiments. * p<0.05, **p<0.005.</p
