<p>(A) Male flies carrying the <i>[w<sup>+</sup> GMroX1]</i> transgene inserted at the 2R telomere (60F) have sectored pigmentation due to dosage compensation at the transgene. (B) Males heterozygous for mutations in <i>Spt5</i> lose most red eye pigmentation due to reduced MSL complex activity. (C and D) <i>Spt5</i> mutants have no effect on the PEV line <i>In(1)w<sup>m4</sup></i>. (E) Genomic location of <i>Spt5</i> and flanking deficiencies. (F) New <i>Spt5</i> mutations failed to complement the previously reported <i>Spt5<sup>MGE-3</sup></i> allele <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003073#pgen.1003073-Mahoney1" target="_blank">[15]</a>. (G) SPT5 domain features. SR, Serine/Arginine; NGN, N-terminal NusG; KOW, Kyrpides, Ouzounis, Woese light green oval indicates only partial match to consensus; RGG, arginine glycine glycine repeats; CTR, C-Terminal Repeats similar to RNAPII. Screen design and results are in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003073#pgen.1003073.s001" target="_blank">Figure S1</a>.</p
