Proteomics Reveals the Effects of Salicylic Acid on
Growth and Tolerance to Subsequent Drought Stress in Wheat
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Abstract
Pretreatment with 0.5 mM salicylic acid (SA) for 3 days significantly
enhanced the growth and tolerance to subsequent drought stress (PEG-6000,
15%) in wheat seedlings, manifesting as increased shoot and root dry
weights, and decreased lipid peroxidation. Total proteins from wheat
leaves exposed to (i) 0.5 mM SA pretreatment, (ii) drought stress,
and (iii) 0.5 mM SA treatment plus drought-stress treatments were
analyzed using a proteomics method. Eighty-two stress-responsive protein
spots showed significant changes, of which 76 were successfully identified
by MALDI-TOF-TOF. Analysis of protein expression patterns revealed
that proteins associated with signal transduction, stress defense,
photosynthesis, carbohydrate metabolism, protein metabolism, and energy
production could by involved in SA-induced growth and drought tolerance
in wheat seedlings. Furthermore, the SA-responsive protein interaction
network revealed 35 key proteins, suggesting that these proteins are
critical for SA-induced tolerance