Foxp3⁺ regulatory T cells in C3aR^−/− mice.

Abstract

<p><i>A</i> and <i>B</i>, Lymphoid cells from the indicated organs were obtained from wild-type and C3aR^−/− mice (<i>A</i>), or the spleens from bone-marrow reconstituted mice (<i>B</i>) generated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052666#pone-0052666-g005" target="_blank"><i>Figure 5</i></a>. The percentage of Foxp3⁺ cells among CD4⁺ (spleen, lung, inguinal lymph node (iLN)) or CD4⁺CD8⁻ (thymus) cells was analyzed by flow cytometry. <i>C</i>, Groups of C3aR^−/− mice were intraperitoneally injected with control IgG or anti-CD25 (PC-61) (n = 3–4) on day −2, day 0, and day 2. The mice were intravenously injected with CD45.1⁺ OT-II T cells on day -1, followed by subsequent intranasal injection with Asp/OVA on day 0, 2, 4, and 6. On day 7, lymphoid cells from the lung or draining lymph nodes were obtained, and the expression of IL-17 by CD4⁺ T cells was analyzed by intracellular staining. Data shown represent three (<i>A</i>) or two (<i>B</i> & <i>C</i>) independent experiments. *, p<0.05 or **, p<0.01 in comparison with WT (<i>A</i>), or isotype control IgG treatment (<i>C</i>).</p