Distal regulatory elements interact with the <i>Lmo2</i> proximal promoter in erythroid cells.

Abstract

<p><b>A</b>) Quantitative chromosome conformation capture (3C) was performed to detect chromatin-chromatin interactions between the 75 DRE (distal regulatory element) upstream of <i>Lmo2</i> and the rest of the <i>Lmo2</i>-<i>Caprin1</i> region of mouse chromosome 2. B) Similarly, 3C was performed to detect chromatin-chromatin interactions between the <i>Lmo2</i> proximal promoter (pP) and distal regulatory elements (DRE). In both the profile of interactions identified in anaemic spleen (red) and kidney (blue) is displayed. Black box indicates the anchor fragment at the 75 DRE or the <i>Lmo2</i> pP and alternating intensities of grey boxes indicate the fragments investigated for interactions. Data points are an average of three to five independent biological replicates. Error bars depict the SEM, * p<0.05, ** p<0.01, and *** p<0.001.</p

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