<p><b>A,</b> Single-cycle infectivity assay of HIV<sub>NL4-3</sub> produced in HEK293T cells transfected with empty vector control (white bars) or V5-tagged A3G (black bars, 120 ng) , 1 µg of NL4-3 proviral DNA and treated with indicated concentrations of MLN4924. <b>B,</b> Parallel immunoblots indicating MLN4924 restores steady-state levels of A3G in cells and packaging in virions. <b>C,</b> Quantitation of G to A mutations in gDNA sequences from virions produced in cells treated with either DMSO or 500 nM MLN4924.</p
