<p>(<b>a</b>) Decrease of Cy5 fluorescence in the process of MNSG4 (formed between oligos <b>Tr<sub>MNS</sub>-III</b> and <b>Comp-Cy5</b>) opened by its C-rich complimentary strand (oligo <b>Tr<sub>MNS</sub>-II</b>) at different temperature. Black, green, blue and cyan curves are from experimental data obtained at 25, 35, 45, and 55°C, respectively, and red curves are fitted results by a double exponential decay model. (<b>b</b>) Decrease of Cy5 fluorescence in the process of −80 G4 (formed between oligos <b>Tr<sub>-80</sub>-III</b> and <b>Comp-Cy5</b>) opened by its C-rich complimentary strand (oligo <b>Tr<sub>-80</sub>-II</b>) at different temperature. Black, green, blue and cyan curves are from experimental data obtained at 35, 45, 55, and 65°C, respectively, and red curves are fitted results by a double exponential decay model. Hybridisation was performed between 100 nM G4 forming oligos and 2 µM complimentary C-rich oligo. The reaction was carried out in 100 mM KCl solution containing 10 mM Tris-HCl (pH 7.4).</p
