<p>(A) Identification of Pax6-binding regions by ChIP-Chip in lens chromatin and a corresponding luciferase reporter construct for transfection assays. (B) A diagrammatic summary of Pax6 mutants, N50K, R128C, R242T and R317X. β, N-terminal β-turn unit; PD, paird domain; PAI, N-termianl subdomian of PD; RED, C-terminal subdomian of PD; L, linker region; HD, homeodomain; PST, proline-serine-threonine rich transactivation domain. (C) Pax6 activates <i>Kif1b</i> promoter in cultured cells. Transient transfections were performed in P19 embryonic carcinoma and in αTN4-1 lens cell as described in Methods. (D) Prediction of Pax6 binding sites with novel Pax6 DNA binding motifs <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0054507#pone.0054507-Xie1" target="_blank">[48]</a>. (E) EMSA validation of Pax6 binding to two probes identified by motifs 2-2 and 4-1. PD/HD, recombinant Pax6 protein containing both Pax6 paired domain (PD) and homeodomain (HD). P6CON, DNA-binding concensus for Pax6 paired domain.</p
