<p>(A) p53 is silenced in MCF-7Vav1 cells following treatment with p53 shRNA. Cells were infected with viral vectors containing shRNA for p53 (shp53) or control (pLKO). Cell lysates were subjected to immunoblotting with anti-p53 antibody. Anti-actin was used as loading control. (B) Real Time PCR analysis of selected apoptosis-related genes. The analysis was performed on the following cells: MCF-7Vector or MCF-7Vav1 infected with control viral vector and MCF-7Vav1 infected with shp53. (C) TUNEL assay of MCF-7Vav1 infected with pLKO or shp53 was performed. Three representative photographs of the cells from different areas are presented. (D) MCF-7Vav1 infected with pLKO or shp53 were suspended in RPMI medium containing 0.3% agar and 10% calf serum, and plated onto a bottom layer containing 0.8% agar. Cells were plated in triplicates, at a density of 1×10<sup>5</sup>/well in a 6-well plate, and colonies were photographed 14 days later. Three independent experiments were analyzed, and representative photographs of cells from different areas are presented.</p
