<p>Cells were probed using affinity purified α-FLA3 rabbit antibodies and a mouse monoclonal antibody against FAZ1 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052846#pone.0052846-Vaughan2" target="_blank">[19]</a> or PFR2 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052846#pone.0052846-Kohl4" target="_blank">[18]</a>. The cells were examined using an Olympus Fluoview 1000 confocal microscope. The panels are presented as merges of the phase and the individual immunofluorescence images plus a combined antibody fluorescence merged image. Bar = 10 µm. Panel A. The location of FLA3 (green), FAZ1 (magenta) and the nucleus/kinetoplast stained with DAPI (blue). Panel B. The location of FLA3 (green), PFR2 (magenta) and the nucleus/kinetoplast (blue). PFR2 but not FLA3 is associated with the flagellum and the free flagellum at the anterior end of the cell (black arrow).</p
