Screening for FEN1 inhibitors <i>in vitro</i>.

Abstract

<p>Schematic representation of the fluorescence-based assay employed to identify FEN1 inhibitors. In the absence of inhibitor, FEN1 cleaves the 5′ flap to which the 6-FAM fluorophore is attached, allowing it to diffuse away from the BHQ-1 quencher and fluoresce. Activity is read as increasing fluorescence over time.</p