The GLS is required for <i>gurken</i> RNA localization and gene function.

Abstract

<p>(A–B) Wild-type expression patterns of endogenous <i>gurken</i> RNA (A) and protein (B) as revealed by whole mount <i>in situ</i> hybridization and immunofluorescence, respectively. Anterodorsal localization of transcripts and protein is only apparent in the rightmost egg chambers, which are stage 8 and 9, respectively. (C–E) The <i>gurken</i> RNA and protein distribution patterns of <i>gurken</i> null mutants (<i>grk^ΔFRT</i>) carrying the wild-type <i>gurken</i> transgene, <i>grk^wt</i> (C–D) or no transgene (E). (F–H) <i>grk^ΔFRT</i> eggs and egg chambers (from <i>gurken</i> null mothers) carrying the <i>grkGLS^mut</i> transgene. (F) Left panel: representative <i>grk^ΔFRT</i>; <i>grkGLS^mut</i> egg exhibiting a completely ventralized phenotype, i.e., complete loss of dorsal appendage material. Right panel; anterior end of a <i>grk^ΔFRT</i>; <i>grkGLS^mut</i> egg exhibiting a strong, but not complete, ventralized phenotype. Note, for example the short, fused dorsal appendage. (G) <i>grk^ΔFRT</i>; <i>grkGLS^mut</i> ovariole following <i>in situ</i> hybridization with <i>gurken</i> probe. Transcripts are dispersed throughout the germ-line cysts with only slight enrichment in the oocyte and no subcellular localization. (H) <i>grk^ΔFRT</i>; <i>grkGLS^mut</i> ovariole following immunofluorescence using an anti-Grk antibody. The protein is generally dispersed throughout the germ-line cysts, although slight enrichment around the oocyte nucleus is seen in rare stage 10 and 11 egg chambers.</p