Psoriasin and MUC1 expression is elevated in CD24⁺ mammary epithelial cells.

Abstract

<p>MCF10A cells were cultured in confluence for 10 days and separated for CD24⁺ cells, using magnetic activated cell sorting. <b>A</b> Psoriasin expression, analyzed by Western blotting, was confined to the CD24⁺ cell fraction, compared with negative selection and controls. Equal loading was confirmed by GAPDH. The figure illustrates a representative example (n = 3). <b>B</b> Separated CD24⁺ cells showed an increase in the expression of CD24 and a decrease in the expression of CD44, compared with negative selection. The expression of MUC1 was increased in the same level as CD24 expression. The figures illustrate representative examples (n = 4).</p